The enhanced standard is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. Two versions of the standard are offered. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods. The molecular weight marker mix also contains a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The SuperSignal Molecular Weight Protein Ladders and SuperSignal Enhanced Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. Thermo Scientificâ„¢ SuperSignal Molecular Weight Protein Ladders (20 to 150KDa) are a ready-to-use mix of recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems.
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